Posted at 10.15.2018
Anti-C1q Antibodies in Patients with Hepatitis B Pathogen Infection
Ahmed Elsadek Fakhr1, Emad Abdelhamid Morad1, Marc van Ranst2, and Mahmoud Reza Pourkarim3
Background: Hepatitis B pathogen (HBV) an infection is associated with extrahepatic manifestations the system which is regarded as immune mediated. One of the autoantibodies accused to be associated with tissues injury in immune intricate disorders is anti-C1q. This may be attributed to the ability of these autoantibodies to amplify match activation in situ. Currently, there are no data describing the prevalence of anti-C1q in patients with HBV illness.
Objectives: The aim of this study was to research the prevalence of anti-C1q antibodies and evaluate possible associations in a inhabitants with HBV contamination.
Materials and Methods: Serum samples were gathered from several 145 patients with HBV disease and 33 obviously healthy settings. Anti-C1q antibodies were quantified by ELISA.
Results: The degrees of anti-C1q antibodies exhibited an extremely statistically significant difference between HBV instances and settings as the mean ± SD were 21. 28 ± 38. 72 and 6. 56 ± 5. 73, respectively (p< 0. 001). Oddly enough, cases with serious HBV infection demonstrated higher anti-C1q levels compared with chronic HBV an infection (35. 37 ± 81. 77 and 20. 43 ± 25. 60, respectively) (p= 0. 031). Levels of anti-C1q antibodies were significantly higher in Belgian and Iranian society versus Egyptian society. Regarding the HBV genotype, the patients enrolled were mainly of genotypes A and D. The levels of anti-C1q antibodies were higher in genotype A (p= 0. 004). Regarding the anti-C1q seropositivity, its prevalence was 22. 8% among HBV situations weighed against 3% among control buttons; however, no significant association has been found with the studied factors.
Conclusions: Patients with HBV infections exhibit increased production of anti-C1q antibodies. This observation may partially explain the tissue damage associated with the extrahepatic involvements of HBV.
Keywords: Anti-C1q antibodies; autoantibodies; Hepatitis; Infection
The aim of this research was to investigate the prevalence of anti-C1q antibodies and assess possible associations in a population with HBV illness.
All types of procedures were conducted in accordance with the ethical principles expressed in the Declaration of Helsinki. Written educated consents were obtained from all subjects signed up for the study.
The analysis was performed as an instance control analysis on 2 communities. A complete of 145 patients with HBV illness were signed up for the first group. From the 145 patients, 65, 64, and 16 were residing in Iran, Belgium, and Egypt, respectively. Patients were categorized into: patients with acute hepatitis B diagnosed by seropositivity for hepatitis B surface antigen (HBs-Ag) and hepatitis B key IgM (HBc-IgM), and patients with serious hepatitis B seen as a existence of HBs-Ag and HBc-IgG. The next group included 33 evidently healthy volunteers. Patients were excluded if indeed they acquired systemic lupus erythematosus (SLE) or co-infected with hepatitis C disease (HCV) or individual immunodeficiency computer virus (HIV). One mL serum was collected from all enrolled topics and stored at -20C till tests.
Anti-C1q persistence in the gathered serum samples was performed using commercial enzyme associated immunosorbent assay set up (QUANTA LiteTM Anti-C1q ELISA, INOVA Diagnostics, Inc. , United states), as per the manufacturer's instructions. The examples were labeled as negative, low positive, moderate positive or strong positive if the anti-C1q principles were <20, 20 - 39, 40 - 80 or >80 units, respectively.
Continuous factors were expressed as the mean ± SD & median (range), and the categorical factors were portrayed as lots. Continuous factors were checked for normality by using Shapiro-Wilk test. Mann Whitney U test was used to compare between two groups of non-normally distributed variables. Kruskal Wallis h test was used to compare between more than two sets of non-normally distributed factors. A p-value <0. 05 was considered significant. All figures were performed using SPSS 22. 0 for house windows (SPSS Inc. , Chicago, IL, USA), MedCalc windows (MedCalc Software bvba 13, Ostend, Belgium) and Microsoft Office Excel 2010 for home windows (Microsoft Cor. , Redmond, WA, USA).